Stereo Dissecting Microscope
all about Stereo Dissecting MicroscopeHuman Skin Cells Reprogrammed Into Embryonic Stem Cells
Human Skin Cells Reprogrammed Into Embryonic Stem Cells
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| • | Gene silencing by RNAi in mouse Sertoli cells |
| Background: RNA interference (RNAi) is a valuable tool in the investigation of gene function. The purpose of this study was to examine the availability, target cell types and efficiency of RNAi in the mouse seminiferous epithelium. Methods: The experimental model was based on transgenic mice expressing EGFP (enhanced green fluorescent protein). RNAi was induced by in vivo transfection of plasmid vectors encoding for short hairpin RNAs (shRNAs) targeting EGFP. shRNAs were transfected in vivo by microinjection into the seminiferous tubules via the rete testis followed by square wave electroporation. As a transfection reporter, expression of red fluorescent protein (HcRed 1) was used. Cell types, the efficiency of both transfections and RNAi were all evaluated. Results: Sertoli cells were the main transfected cells. A reduction of about 40% in the level of EGFP protein was detected in cells successfully transfected both in vivo and in vitro. However, the efficiency of in vivo transfection was low. Conclusion: In adult seminiferous epithelial cells, in vivo post-transcriptional gene silencing mediated by RNAi via shRNA is efficient in Sertoli cells. Similar levels of RNAi were detected both in vivo and in vitro. This also indicates that Sertoli cells have the necessary silencing machinery to repress the expression of endogenous genes via RNAi. | |
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| • | The 2008 Garrod Lecture: Antimicrobial resistance--animals and the environment |
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The evolution of resistance to microbes is one of the most significant problems in modern medicine, posing serious threats to human and animal health. The early work on the use of antibiotics to bacterial infections gave much hope that infectious diseases were no longer a problem, especially in the human field. However, as their use, indeed over-use, progressed, resistance (both monoresistance and multiresistance), which was often transferable between different strains and species of bacteria, emerged. In addition, the situation is increasingly complex, as various mechanisms of resistance, including a wide range of β-lactamases, are now complicating the issue. The use of antibiotics in animals, especially those used for growth promotion, has come in for serious criticism, especially those where their use should be reserved for difficult human infections. To lend control, certain antibiotic growth promoters have been banned from use in the EU and the UK. Antimicrobial resistance is not confined to bacteria but occurs in viruses, protozoa and helminths. In many of these, the mechanism of resistance is unknown, and hence their control is still in question. It is likely, however, that the mechanisms are no less complicated than those pertaining to bacteria. |
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| • | Nanotech: A regulatory blueprint for the next administration |
| Nanotechnology will significantly change virtually every facet of the way we live. The next president has the opportunity to shape these changes and to ensure that nanotechnology's benefits will be maximized and its risks identified and controlled. (2008-07-23) | |
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| • | Alcohol Use Frequently Overlooked Before Surgery |
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| • | Ancient Rain Forest Insects |
| AmberDepot provides the largest selection of fossil amber on the Internet. We have been a top seller of fossil insects in amber for many years. We sell both retail and wholesale to customers around the world. | |
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| • | Microscope World, Microscopes for every application |
| Measuring microscopes - Stereo microscopes - Polarizing microscopes - Inverted microscopes - Metallurgical microscope - Asbestos microscope | |
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