Stereo Dissecting Microscope

all about Stereo Dissecting Microscope
Stereo Dissecting Microscope Resources

Home > Stereo Dissecting Microscope > Gene silencing by RNAi in mouse Sertoli cells

Gene silencing by RNAi in mouse Sertoli cells




Background: RNA interference (RNAi) is a valuable tool in the investigation of gene function. The purpose of this study was to examine the availability, target cell types and efficiency of RNAi in the mouse seminiferous epithelium. Methods: The experimental model was based on transgenic mice expressing EGFP (enhanced green fluorescent protein). RNAi was induced by in vivo transfection of plasmid vectors encoding for short hairpin RNAs (shRNAs) targeting EGFP. shRNAs were transfected in vivo by microinjection into the seminiferous tubules via the rete testis followed by square wave electroporation. As a transfection reporter, expression of red fluorescent protein (HcRed 1) was used. Cell types, the efficiency of both transfections and RNAi were all evaluated. Results: Sertoli cells were the main transfected cells. A reduction of about 40% in the level of EGFP protein was detected in cells successfully transfected both in vivo and in vitro. However, the efficiency of in vivo transfection was low. Conclusion: In adult seminiferous epithelial cells, in vivo post-transcriptional gene silencing mediated by RNAi via shRNA is efficient in Sertoli cells. Similar levels of RNAi were detected both in vivo and in vitro. This also indicates that Sertoli cells have the necessary silencing machinery to repress the expression of endogenous genes via RNAi.


Click Here to read the entire article...




Other Articles:


Medicine From Milk: Gene Therapy Could Transform Goats Into Pharmaceutical Factories
Gene therapy has been used successfully to breed large animals capable of producing therapeutic proteins in their milk, such as insulin or those that fight cancer. This represents a significant milestone, as previous methods involved cloning, which takes more time and generally costs more. This new research should reduce the cost and increase the availability of several drugs.
Click Here to read the entire article...
The 2008 Garrod Lecture: Antimicrobial resistance--animals and the environment

The evolution of resistance to microbes is one of the most significant problems in modern medicine, posing serious threats to human and animal health. The early work on the use of antibiotics to bacterial infections gave much hope that infectious diseases were no longer a problem, especially in the human field. However, as their use, indeed over-use, progressed, resistance (both monoresistance and multiresistance), which was often transferable between different strains and species of bacteria, emerged. In addition, the situation is increasingly complex, as various mechanisms of resistance, including a wide range of β-lactamases, are now complicating the issue.

The use of antibiotics in animals, especially those used for growth promotion, has come in for serious criticism, especially those where their use should be reserved for difficult human infections. To lend control, certain antibiotic growth promoters have been banned from use in the EU and the UK.

Antimicrobial resistance is not confined to bacteria but occurs in viruses, protozoa and helminths. In many of these, the mechanism of resistance is unknown, and hence their control is still in question. It is likely, however, that the mechanisms are no less complicated than those pertaining to bacteria.

Click Here to read the entire article...
Nanotech: A regulatory blueprint for the next administration
Nanotechnology will significantly change virtually every facet of the way we live. The next president has the opportunity to shape these changes and to ensure that nanotechnology's benefits will be maximized and its risks identified and controlled. (2008-07-23)
Click Here to read the entire article...
Alcohol Use Frequently Overlooked Before Surgery
Click Here to read the entire article...
Ancient Rain Forest Insects
AmberDepot provides the largest selection of fossil amber on the Internet. We have been a top seller of fossil insects in amber for many years. We sell both retail and wholesale to customers around the world.
Click Here to read the entire article...
Microscope World, Microscopes for every application
Measuring microscopes - Stereo microscopes - Polarizing microscopes - Inverted microscopes - Metallurgical microscope - Asbestos microscope
Click Here to read the entire article...
Reproductive Biology and Endocrinology - Latest articles
- Copyright © 2008 Microsoft. All rights reserved. These XML results may not be used, reproduced or transmitted in any manner or for any purpose other than rendering Live Search results within an RSS aggregator for your personal, non-commercial use. Any other use of these results requires express written permission from Microsoft Corporation. By accessing this web page or using these results in any manner whatsoever, you agree to be bound by the foregoing restrictions. - version: v1.5 build A